Applied Fluorescence and Microscopy in Biophysics

Shreya Pramanik, Agustin Mangiarotti, Rumiana Dimova (MPIKG, CC)

This course will start with an introduction to fluorescence and gradually advance towards using it for modern-day research, with a maximum emphasis on biophysical studies. We will talk about the different applications of fluorescence that can be used for experiments in bulk studies (as in a fluorimeter) and on individual objects (as in microscopy). The attendants will be welcome to see our microscope facilities for hands-on experience with the acquired knowledge.

This would be an interactive course using slides and hand drawings on boards.

Covered topics:

·       Introduction to fluorescence and how to harness for biophysics

·       Lasers (types and principle of work); Optical tweezers; Light-matter interaction

·       Confocal microscope; Two-photon microscope; Second harmonic generation

·      -Different types of super resolution microscopy

·       General polarization; Dye orientation; Fluorescence life-time imaging microscopy (FLIM)

·       Recap after holidays; Light-sheet microscopy

·       Fluorescence correlation spectroscopy (FCS); Raster image correlation spectroscopy (RICS); Fluorescence recovery after photobleaching (FRAP)

·       Förster resonance energy transfer (FRET); Quenching; Total internal reflection fluorescence (TIRF); Mass interferometry

·       Other kinds of imaging microscopy: differential interference contrast (DIC), phase contrast, dark field, bright field

·       Recap of whole course + discussions of latest papers on the techniques learnt

Please contact Shreya Pramanik Shreya.Pramanik@mpikg.mpg.de and Agustin Mangiarotti Agustin.Mangiarotti@mpikg.mpg.de for more information.